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Experimental Protocol for Detecting Mitochondrial Function in Hepatocytes Exposed to Organochlorine Pesticides
1State Key Laboratory of Reproductive Medicine, Institute of Toxicology,Nanjing Medical University, 2Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health,Nanjing Medical University, 3Center of Gallbladder Disease, Shanghai East Hospital, Institute of Gallstone Disease,Tongji University School of Medicine
Kapitel
- 00:04Titel
- 00:36Mitochondrial Fluorescence Intensity and Cellular ATP Detection
- 02:45Mitochondrial Membrane Potential Measurements
- 04:39Oxygen Consumption Rate (OCR) Measurements
- 06:22Results: Evaluation of Mitochondrial Function in Hepatocytes Exposed to β-HCH
- 07:43Conclusion
Understanding the influence of environmental organochlorine pesticides (OCPs) on mitochondrial function in hepatocytes is important in exploring the mechanism of OCPs causing metabolic disorders. This paper presents detailed methods on detecting hepatic mitochondrial function.
