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Detecção rápida de desenvolvimento neurológico fenótipos nas células precursoras neurais humanas (NPCs)
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells (NPCs)

Detecção rápida de desenvolvimento neurológico fenótipos nas células precursoras neurais humanas (NPCs)

9,797 Views

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10:47 min

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March 02, 2018

DOI:

10.3791/56628-v

DOI:
10.3791/56628-v

•

10:47 min
March 02, 2018

•

8 Views
, , , , , , , , ,
1Department of Neuroscience and Cell Biology,Rutgers Robert Wood Johnson Medical School, 2Center for Advanced Biotechnology and Medicine, Department of Neuroscience and Cell Biology,Rutgers Robert Wood Johnson Medical School, 3The Child Health Institute of NJ, Department of Obstetrics, Gynecology, and Reproductive Services,Rutgers Robert Wood Johnson Medical School, 4The Child Health Institute of NJ, Department of Neuroscience and Cell Biology,Rutgers Robert Wood Johnson Medical School, 5Department of Genetics,Rutgers University

Summary

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Desenvolvimento neurológico processos tais como consequência natural do axônio, migração e proliferação são frequentemente perturbadas em doenças neuropsiquiátricas. Assim, apresentamos protocolos para rapidamente e reproducibly avaliar estes processos de desenvolvimento neurológico em NPCs de iPSC-derivado humanos. Estes protocolos permitem também a avaliação dos efeitos da terapêutica e fatores de crescimento relevantes no desenvolvimento do NPC.

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Williams, M., Prem, S., Zhou, X., Matteson, P., Yeung, P. L., Lu, C., Pang, Z., Brzustowicz, L., Millonig, J. H., Dicicco-Bloom, E. Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells (NPCs). J. Vis. Exp. (133), e56628, doi:10.3791/56628 (2018).

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