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Um método simples e eficiente para estabelecer linhas de células T de pacientes com infecção crônica ativa vírus Epstein - Barr e NK
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Um método simples e eficiente para estabelecer linhas de células T de pacientes com infecção crônica ativa vírus Epstein - Barr e NK
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March 30, 2018
DOI:
1Genetic Resources Center, Institute of Genetics and Developmental Biology,Chinese Academy of Sciences, 2MOE Key Laboratory of Major Diseases in Children, National Key Discipline of Pediatrics, National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children’s Hospital,Capital Medical University, 3State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology,Chinese Academy of Sciences
Summary
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Um método simples para a obtenção de clones NK e células T de pacientes CAEBV foi desenvolvido com alta eficiência, uma pequena quantidade de sangue periférico e uma baixa dose de Il-2.
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Xu, C., Ai, J., Zhang, Q., Li, T., Wu, X., Xie, Z., Duan, Z. An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection. J. Vis. Exp. (133), e56515, doi:10.3791/56515 (2018).
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