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Kvantitative hele-mount immunofluorescens analyse af Cardiac stamfader populationer i mus embryoner
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1Cell, Developmental, and Regenerative Biology Department,Icahn School of Medicine at Mount Sinai, 2Mindich Child Health and Development Institute,Icahn School of Medicine at Mount Sinai, 3Black Family Stem Cell Institute,Icahn School of Medicine at Mount Sinai, 4Microscopy Core,Icahn School of Medicine at Mount Sinai, 5Department of Neuroscience,Icahn School of Medicine at Mount Sinai, 6Friedman Brain Institute,Icahn School of Medicine at Mount Sinai
Kapitel
- 00:05Titel
- 00:55Cardiac Crescent Stage Embryo Harvest and Processing
- 02:45Immunofluorescence Staining
- 03:57Embryo Mounting and Confocal Imaging
- 05:28Image Analysis and Quantitative 3D Modeling
- 07:49Results: Representative Confocal Image Processing
- 08:56Conclusion
Her, beskriver vi en protokol for hele mount immunofluorescens og image-baserede kvantitative volumetrisk analyse af tidlige fase mus embryoner. Vi præsenterer denne teknik som en kraftfuld tilgang til kvalitativt og kvantitativt vurdere hjerte strukturer under udvikling, og foreslår, at det kan være meget tilpasses andre organsystemer.
