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高通量并行测序在金黄叙利亚仓鼠感染期间测量钩端螺旋体螺旋体转插入突变体的适应性
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11:50 min
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December 18, 2017
DOI:
1Veterans Affairs Greater Los Angeles Healthcare System, 2Departments of Medicine,David Geffen School of Medicine at University of California Los Angeles, 3Departments of Urology,David Geffen School of Medicine at University of California Los Angeles, 4Departments of Microbiology, Immunology, and Molecular Genetics,University of California Los Angeles
Summary
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我们在这里描述的技术, 结合转诱变和高通量测序, 以确定和量化转端突变体在组织后的挑战, 仓鼠。该协议可用于筛选动物生存和传播的变种人, 也可用于体外研究。
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Cite this Article
Lourdault, K., Matsunaga, J., Evangelista, K. V., Haake, D. A. High-throughput Parallel Sequencing to Measure Fitness of Leptospira interrogans Transposon Insertion Mutants During Golden Syrian Hamster Infection. J. Vis. Exp. (130), e56442, doi:10.3791/56442 (2017).
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