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Modified Roller Tube Method for Precisely Localized and Repetitive Intermittent Imaging During Long-term Culture of Brain Slices in an Enclosed System
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JoVE Journal Neurowissenschaften
Modified Roller Tube Method for Precisely Localized and Repetitive Intermittent Imaging During Long-term Culture of Brain Slices in an Enclosed System

Modified Roller Tube Method for Precisely Localized and Repetitive Intermittent Imaging During Long-term Culture of Brain Slices in an Enclosed System

10,499 Views

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09:52 min

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December 28, 2017

DOI:

10.3791/56436-v

DOI:
10.3791/56436-v

•

09:52 min
December 28, 2017

•

2 Views
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1Department of Biochemistry and Molecular Biology and Molecular, Cellular and Integrated Neuroscience Program,Colorado State University, 2IBMC-Instituto de Biologia Molecular e Celular, i3S-Instituto de Investigaçãoe Inovação em Saúde, ICBAS,Universidade do Porto, 3Denali Therapeutics

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Presented here is a modified roller tube method for culturing and intermittent high-resolution imaging of rodent brain slices over many weeks with precise repositioning on photoetched coverslips. Neuronal viability and slice morphology are well maintained. Applications of this fully enclosed system using viruses for cell-type specific expression are provided.

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Fixman, B. B., Babcock, I. W., Minamide, L. S., Shaw, A. E., Oliveira da Silva, M. I., Runyan, A. M., Maloney, M. T., Field, J. J., Bamburg, J. R. Modified Roller Tube Method for Precisely Localized and Repetitive Intermittent Imaging During Long-term Culture of Brain Slices in an Enclosed System. J. Vis. Exp. (130), e56436, doi:10.3791/56436 (2017).

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