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实时颤振诱发的粪便中 CWD 朊的转化检测
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material
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实时颤振诱发的粪便中 CWD 朊的转化检测

13,328 Views

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09:50 min

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September 29, 2017

DOI:

10.3791/56373-v

DOI:
10.3791/56373-v

•

09:50 min
September 29, 2017

•

10 Views
, , , , ,
1Dept. of Ecosystem and Public Health, Calgary Prion Research Units, Faculty of Veterinary Medicine,University of Calgary, 2Dept. of Molecular Biology,University of Wyoming, 3Canadian Food Inspection Agency,Lethbridge Laboratories

Summary

Automatically generated

在这里, 我们提出一个协议来描述一个简单, 快速和有效的朊病毒放大技术, real-time 颤抖诱导转换 (RT-QuIC) 方法。

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Cite this Article

Cheng, Y. C., Hannaoui, S., John, T. R., Dudas, S., Czub, S., Gilch, S. Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material. J. Vis. Exp. (127), e56373, doi:10.3791/56373 (2017).

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