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Bioprinting of Cartilage and Skin Tissue Analogs Utilizing a Novel Passive Mixing Unit Technique for Bioink Precellularization
JoVE Journal
Biotechnik
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JoVE Journal Biotechnik
Bioprinting of Cartilage and Skin Tissue Analogs Utilizing a Novel Passive Mixing Unit Technique for Bioink Precellularization

Bioprinting of Cartilage and Skin Tissue Analogs Utilizing a Novel Passive Mixing Unit Technique for Bioink Precellularization

DOI:
10.3791/56372-v

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09:03 min

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January 03, 2018

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, ,
1CELLINK LLC, 2CELLINK AB, 33D Bioprinting Centre, Department of Chemistry and Chemical Engineering,Chalmers University of Technology

Kapitel

  • 00:05Titel
  • 00:51Preparation of Consumables, Bioink, and Cells
  • 02:33Mixing of Cell Suspension and Bioink
  • 04:44Bioprinting of Cartilage Analogs with a Single Cell type
  • 07:28Results: Cell viability after Mixing and During Long-term Culture
  • 08:27Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Cartilage and skin analogs were bioprinted using a nanocellulose-alginate based bioink. The bioinks were cellularized prior to printing via a single step passive mixing unit. The constructs were demonstrated to be uniformly cellularized, have high viability, and exhibit favorable markers of differentiation.

Tags

BioprintingCartilagePassive Mixing UnitBioink PrecellularizationCell ViabilityMixing RatioNanocellulose AlginateCalcium ChlorideCell SuspensionTrypan BlueCell DensityFibroblastsDispensing UnitLuer Lock

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