Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli
9,121 Views
•
10:34 min
•
August 22, 2017
DOI:
Summary
Automatically generated
Here we present a method for directly measuring transfer RNA charging levels from purified Escherichia coli RNA as well as a way to compare relative levels of transfer RNA, or any other short RNA, across different samples based on the addition of spike-in cells expressing a reference gene.
Read Article
Cite this Article
Stenum, T. S., Sørensen, M. A., Svenningsen, S. L. Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli. J. Vis. Exp. (126), e56212, doi:10.3791/56212 (2017).
Copy