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Determination of the Glycogen Content in Cyanobacteria
JoVE Journal
Biochemie
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JoVE Journal Biochemie
Determination of the Glycogen Content in Cyanobacteria
DOI:

07:04 min

July 17, 2017

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Kapitel

  • 00:05Titel
  • 01:01Lysis of Cyanobacterial Cells
  • 02:41Glycogen Precipitation, Enzymatic Hydrolysis, and Glycogen Determination
  • 04:30Determination of Total Glucose Content by the Glucose Oxidase-peroxidase (GOD-POD) Reagent
  • 05:30Results: Measurement of Glycogen Contents in Synechocystis sp.
  • 06:15Conclusion

Summary

Automatische Übersetzung

Here, we present a reliable and easy assay to measure the glycogen content in cyanobacterial cells. The procedure entails precipitation, selectable depolymerization, and the detection of glucose residues. This method is suitable for both wildtype and genetically engineered strains and can facilitate the metabolic engineering of cyanobacteria.

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