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Immunology and Infection

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JoVE Journal Immunology and Infection
Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
 

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Article DOI: 10.3791/55824-v • 12:50 min • September 15th, 2017
September 15th, 2017 •
1, 1, 1, *1, *1
1Pharmacological Therapies Unit, Research Institute for Rare Diseases, Institute of Health Carlos III
* These authors contributed equally

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Summary
Automatic Translation

Vascular endothelium tightly controls leukocyte recruitment. Inadequate leukocyte extravasation contributes to human inflammatory diseases. Therefore, searching for novel regulatory elements of endothelial activation is necessary to design improved therapies for inflammatory disorders. Here, we describe a comprehensive methodology to characterize novel endothelial regulators that can modify leukocyte trafficking during inflammation.

Tags

Keywords: Endothelial Regulators Inflammatory Response Screening Assay Inflammatory Disorders Leukocyte Recruitment Cytokines Chemokines Adhesion Molecules RT-qPCR Wes MLEC-04 Cells Lipopolysaccharide (LPS) Ccl5 Cxcl1 Cxcl10 E-selectin VCAM-1 ICAM-1
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