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Mixed Primary Cultures of Murine Small Intestine Intended for the Study of Gut Hormone Secretion and Live Cell Imaging of Enteroendocrine Cells
JoVE Journal
Biologie
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JoVE Journal Biologie
Mixed Primary Cultures of Murine Small Intestine Intended for the Study of Gut Hormone Secretion and Live Cell Imaging of Enteroendocrine Cells

Mixed Primary Cultures of Murine Small Intestine Intended for the Study of Gut Hormone Secretion and Live Cell Imaging of Enteroendocrine Cells

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09:16 min

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April 20, 2017

DOI:

10.3791/55687-v

DOI:
10.3791/55687-v

•

09:16 min
April 20, 2017

•

9 Views
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1Metabolic Research Laboratories and MRC Metabolic Diseases Unit,Wellcome Trust-MRC Institute of Metabolic Science, University of Cambridge

Summary

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This protocol describes the isolation and culture of mixed murine small intestinal cells. These primary intestinal cultures enable the investigation of signaling pathways underlying gut peptide secretion using a number of techniques.

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Enteroendocrine CellsGut Hormone SecretionLive Cell ImagingMixed Primary CulturesMurine Small IntestineIntestinal EpitheliumIntestinal ContentsMuscle LayerEpitheliumChymeMucus

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Cite this Article

Psichas, A., Tolhurst, G., Brighton, C. A., Gribble, F. M., Reimann, F. Mixed Primary Cultures of Murine Small Intestine Intended for the Study of Gut Hormone Secretion and Live Cell Imaging of Enteroendocrine Cells. J. Vis. Exp. (122), e55687, doi:10.3791/55687 (2017).

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