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Analiz için Protein-tRNA Agaroz Jel Retardasyon Testleri<em> K</em<sup> 6</sup> Threonylcarbamoyladenosine TcdA Function
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Biochemie
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JoVE Journal Biochemie
Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function
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Analiz için Protein-tRNA Agaroz Jel Retardasyon Testleri<em> K</em<sup> 6</sup> Threonylcarbamoyladenosine TcdA Function

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08:03 min

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June 21, 2017

DOI:

10.3791/55638-v

DOI:
10.3791/55638-v

•

08:03 min
June 21, 2017

•

10 Views
, , , ,
1Department of Physical Chemical Biology, Center for Biological Research (CIB-CSIC),Spanish National Research Council (CSIC), 2Department of Immunology,Complutense University School of Medicine, 3Abvance Biotech srl

Summary

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TRNA (UUU) üretimi ve agaroz jel geciktirme testleri ile TcdA enzimi ile kompleks içinde tRNA (UUU) analizi için bir protokol sunulmuştur.

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Cite this Article

Fernández, F. J., Gómez, S., Navas-Yuste, S., López-Estepa, M., Vega, M. C. Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function. J. Vis. Exp. (124), e55638, doi:10.3791/55638 (2017).

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