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蛋白质tRNA琼脂糖凝胶延迟测定分析<em>ñ</em<sup> 6</sup> - 磺酰氨基甲酰腺苷TcdA功能
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Biochemie
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JoVE Journal Biochemie
Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function
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蛋白质tRNA琼脂糖凝胶延迟测定分析<em>ñ</em<sup> 6</sup> - 磺酰氨基甲酰腺苷TcdA功能

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08:03 min

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June 21, 2017

DOI:

10.3791/55638-v

DOI:
10.3791/55638-v

•

08:03 min
June 21, 2017

•

10 Views
, , , ,
1Department of Physical Chemical Biology, Center for Biological Research (CIB-CSIC),Spanish National Research Council (CSIC), 2Department of Immunology,Complutense University School of Medicine, 3Abvance Biotech srl

Summary

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介绍了通过琼脂糖凝胶阻滞测定技术生产tRNA（UUU）和tDNA（UUU）与酶TcdA复合物的分析方案。

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Cite this Article

Fernández, F. J., Gómez, S., Navas-Yuste, S., López-Estepa, M., Vega, M. C. Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function. J. Vis. Exp. (124), e55638, doi:10.3791/55638 (2017).

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