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Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques
JoVE Journal
Medizin
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JoVE Journal Medizin
Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques

Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques

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11:06 min

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May 02, 2017

DOI:

10.3791/55617-v

DOI:
10.3791/55617-v

•

11:06 min
May 02, 2017

•

3 Views
, , , , , , , , , ,
1Department of Pharmaceutics,Washington National Primate Research Center, University of Washington, 2Division of Primate Resources,Washington National Primate Research Center, University of Washington

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Here, we describe a minimally invasive laparoscopic technique for serial sampling of liver and mesenteric lymph nodes (MLN) in macaques that allows for increased sampling frequency, and reduces the potential for surgical complications when compared to performing a laparotomy.

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Laparoscopic TechniqueSerial CollectionLiverMesenteric Lymph NodesMacaquesMinimally InvasiveGI TractMicrobial TranslocationHIV BiologyImmunological ConsequencesPathologyMultiple Time PointsAnimal WelfareSterile ProcedureScalpel IncisionVarus NeedlePneumoperitoneumCO2 Insufflator

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Zevin, A. S., Moats, C., May, D., Wangari, S., Miller, C., Ahrens, J., Iwayama, N., Brown, M., Bratt, D., Klatt, N. R., Smedley, J. Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques. J. Vis. Exp. (123), e55617, doi:10.3791/55617 (2017).

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