/

Immunologie und Infektion

/

慢性自身免疫性干眼鼠模型增加效应记忆T细胞在眼球组织

JoVE Journal
Immunologie und Infektion

This content is Free Access.

JoVE Journal Immunologie und Infektion

A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue

Please note that all translations are automatically generated. Click here for the English version.

慢性自身免疫性干眼鼠模型增加效应记忆T细胞在眼球组织

DOI:

10.3791/55592-v

•

09:42 min

•

June 07, 2017

•

Aihua Hou², Tanima Bose, K. George Chandy, Louis Tong^2,4,5

¹Singapore Eye Research Insitute, A Member of SingHealth, ²DUKE-National University of Singapore Medical School, ³Lee Kong Chian School of Medicine,Nanyang Technological University, ⁴Singapore National Eye Center, ⁵Yong Loo Lin School of Medicine,National University of Singapore

Kapitel

00:05Titel
01:18Preparation of Lacrimal Gland Extract
02:32Preparation of Emulsion
03:30Immunization of Lewis Rats and Injection of Antigen Mixture into the Forniceal Subconjunctiva and Lacrimal Gland
04:53Assessment of Dry Eye Features
07:19Ergebnisse
08:57Conclusion

Summary

Automatische Übersetzung

该报告描述了通过用大鼠泪腺提取物，卵清蛋白和完全弗氏佐剂的乳液免疫在Lewis大鼠中诱导慢性实验性自身免疫性干眼的方法，随后将泪腺提取物和卵清蛋白注射入穹隆结膜下和泪腺六周后。

Tags

Keywords: Autoimmune Dry Eye Rat Model Lacrimal Gland Effector Memory T Cells Ocular Disease Lymphocyte Trafficking Sprague Dawley Rat Lacrimal Gland Extract Freund’s Adjuvant Ovalbumin

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

T细胞受体在小鼠T细胞的逆转录病毒转导

免疫细胞在受伤的脊髓组织流式细胞仪定量评估：一个细胞的分离纯化方法的新颖使用

从初级人类的幼稚和记忆T细胞的诱导调节性T细胞的生成

piggyBac转座子初级人类T细胞系统改造

神经干/祖细胞在小鼠EAE的慢性全身性注射

诱导小鼠肠道炎症由效应CD4的过继转移<sup> +</sup> CD45RB<sup>高</sup> T细胞免疫缺陷小鼠

长期培养的干扰素γ酶联免疫检测方法的评估效应和记忆T细胞应答的牛应用

分析<em>小肠结肠炎耶尔森菌</em>效应易位到宿主细胞中使用β-内酰胺酶效应融合体

诱导麻痹及视觉系统损伤小鼠 T 细胞特异的视神经脊髓炎抗原水通道蛋白-4

慢性活性泼斯坦-巴尔病毒感染患者 NK 和 T 细胞系建立的高效简便方法

Read Article