02:49CYP Activity Quantification in Cell Cultures: Metabolic Probes, Inducers, and Inhibitors
04:30CYP Activity Quantification in Cell Cultures: Medium Treatment
05:40CYP Activity Quantification in Cell Cultures: Mass Spectrometry (MS)-based Quantification
06:49CYP Activity Quantification in Cell Cultures: Probes Using Luminescence-based Methods
08:10Results: Quantification of Metabolites Produced by tha Activity of CYP Enzymes Following Cell Incubation with CYP Inducers, Substrates, and Inhibitors
Metabolic kompetens in vitro-system är en viktig förutsättning för biotransformeringen och disposition av droger och gifter. I detta protokoll beskriver vi tillämpningen av referens metaboliska sönder för att bedöma fas I metabolism i cellodlingar.