02:49CYP Activity Quantification in Cell Cultures: Metabolic Probes, Inducers, and Inhibitors
04:30CYP Activity Quantification in Cell Cultures: Medium Treatment
05:40CYP Activity Quantification in Cell Cultures: Mass Spectrometry (MS)-based Quantification
06:49CYP Activity Quantification in Cell Cultures: Probes Using Luminescence-based Methods
08:10Results: Quantification of Metabolites Produced by tha Activity of CYP Enzymes Following Cell Incubation with CYP Inducers, Substrates, and Inhibitors
Metabolsk kompetansen av in vitro-systemer er en viktig forutsetning for biotransformasjonen og disponering av narkotika og giftstoffer. I denne protokollen beskriver vi anvendelse av referanse metabolske prober for å vurdere fase I metabolisme i cellekulturer.