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为了应对抑菌活性的实时成像人力初级中性粒细胞和单核细胞<em>一种。曲霉</em
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus
DOI:

12:29 min

April 19, 2017

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Kapitel

  • 00:05Titel
  • 00:54Aspergillus fumigatus Culture and Labeling
  • 04:28Isolation of Human Monocytes
  • 07:30Isolation of Human Neutrophils
  • 08:30Live Cell Video Microscopy
  • 09:31Results: In Vitro Evaluation of Primary Immune Response to Aspergillus Conidia
  • 11:11Conclusion

Summary

Automatische Übersetzung

在这里,我们描述了一种协议与活细胞视频显微结合使用荧光曲霉分生孢子记者评估初级人类免疫细胞的抗真菌活性在实时和流式细胞术。所生成的数据提供深入了解宿主细胞曲霉相互作用如杀真菌活性,吞噬作用,细胞迁移和真菌生长的抑制。

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