Journal
/
Biochemie
/
Geliştirilmiş Numune Kombine Öncül İzotop Etiketleme ve Isobaric Etiketlemeyi Kullanma Dokuların çoğullama (cPILOT)
JoVE Journal
Biochemie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Biochemie
Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
Please note that all translations are automatically generated. Click here for the English version.

Geliştirilmiş Numune Kombine Öncül İzotop Etiketleme ve Isobaric Etiketlemeyi Kullanma Dokuların çoğullama (cPILOT)

6,749 Views

•

09:06 min

•

May 01, 2017

DOI:

10.3791/55406-v

DOI:
10.3791/55406-v

•

09:06 min
May 01, 2017

•

5 Views
, , , ,
1Department of Chemistry,University of Pittsburgh, 2SGS North America Inc., 3Large Molecule Analytical Development, Pharmaceutical Development & Manufacturing Science,Janssen Research and Development

Summary

Automatically generated

Kombine ön-madde izotopik etiketleme ve izobarik etiketleme (cPILOT) izobarik etiketlerin örnek çoğullama kabiliyetini arttıran bir sayısal proteomik stratejisidir. Bu protokol bir Alzheimer hastalığı fare modeli ve vahşi tipli kontrollerden dokulara cPILOT uygulanmasını tarif etmektedir.

Explore More Videos

Enhanced Sample MultiplexingCombined Precursor Isotopic LabelingIsobaric TaggingCPILOTAlzheimer’s DiseaseMouse ModelProtein ChangesAgingNeurodegenerative DiseaseCancerPathogenesisDiagnosisPrognosisTherapeutic TargetsAcetylationProtein NitrationBrainHeartLiverBCA AssayProtein ConcentrationDithiothreitolIodoacetamideL-cysteineTrypsin Digestion

Read Article

Cite this Article

King, C. D., Dudenhoeffer, J. D., Gu, L., Evans, A. R., Robinson, R. A. S. Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT). J. Vis. Exp. (123), e55406, doi:10.3791/55406 (2017).

Download .ris file

Copy

Share Video

.

Copy