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用于表征土壤微生物的脂质提取和分析方法在许多样品的实验中

JoVE Journal
Umwelt
Author Produced

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JoVE Journal Umwelt

A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples

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用于表征土壤微生物的脂质提取和分析方法在许多样品的实验中

19,131 Views

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17:39 min

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July 16, 2017

DOI:

10.3791/55310-v

DOI:

10.3791/55310-v

•

17:39 min

July 16, 2017

•

5 Views

Lawrence G. Oates¹, Harry W. Read², Jessica L. M. Gutknecht³, David S. Duncan¹, Teri B. Balser⁴, Randall D. Jackson¹

¹Department of Agronomy and Great Lakes Bioenergy Research Center,University of Wisconsin – Madison, ²Department of Soil Science,University of Wisconsin – Madison, ³Department of Soil, Water, and Climate,University of Minnesota, ⁴Faculty of Science and Engineering,Curtin University

Summary

该文章描述了一种增加产量，同时平衡从微生物细胞膜提取脂质的努力和准确性，用于表征总脂质和指示性脂质的相对丰度，以确定具有许多样品的研究中的土壤微生物群落结构。

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