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生きたゼブラフィッシュ胚における運動ニューロン膜電位のバイオセンシング
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos
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生きたゼブラフィッシュ胚における運動ニューロン膜電位のバイオセンシング

6,503 Views

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10:18 min

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June 26, 2017

DOI:

10.3791/55297-v

DOI:
10.3791/55297-v

•

10:18 min
June 26, 2017

•

14 Views
, , , ,
1Department of Medical Biotechnology and Translational Medicine,Università degli Studi di Milano, 2Department of Neuroscience; Department of Cell Biology,Howard Hughes Medical Institute, Yale University School of Medicine, 3Program in Cellular Neuroscience, Neurodegeneration and Repair,Yale University School of Medicine, 4Department of BioSciences,Università degli Studi di Milano

Tags

BiosensingMotor NeuronMembrane PotentialLive Zebrafish EmbryosFRET-based GEVINoninvasiveExcitable CellsNeuronsNeurological DisordersMermaid BiosensorHuC PromoterPCRRestriction DigestionDNA LigationTransformation

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Cite this Article

Benedetti, L., Ghilardi, A., Prosperi, L., Francolini, M., Del Giacco, L. Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos. J. Vis. Exp. (124), e55297, doi:10.3791/55297 (2017).

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