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Immunologie und Infektion
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Legionella pneumophila exterior vesículas de membrana: Aislamiento y análisis de su potencial pro-inflamatoria en los macrófagos
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages
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Legionella pneumophila exterior vesículas de membrana: Aislamiento y análisis de su potencial pro-inflamatoria en los macrófagos

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08:34 min

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February 22, 2017

DOI:

10.3791/55146-v

DOI:
10.3791/55146-v

•

08:34 min
February 22, 2017

•

9 Views
, , , , ,
1Institute for Lung Research, Universities of Giessen and Marburg Lung Center,Philipps-University Marburg, 2German Center for Lung Research, 3Department of Medicine, Pulmonary and Critical Care Medicine,University Medical Center Giessen and Marburg

Summary

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A continuación, se describe la purificación de Legionella pneumophila (L. pneumophila) vesículas de membrana externa (OMV) a partir de cultivos líquidos. Estas vesículas purificadas se usan después para el tratamiento de los macrófagos para analizar su potencial pro-inflamatoria.

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Cite this Article

Jung, A. L., Hoffmann, K., Herkt, C. E., Schulz, C., Bertrams, W., Schmeck, B. Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages. J. Vis. Exp. (120), e55146, doi:10.3791/55146 (2017).

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