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Legionella pneumophila ytre membran Blemmer: Isolering og analyse av deres Pro-inflammatorisk potensialet på Makrofager
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages
DOI:

08:34 min

February 22, 2017

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Kapitel

  • 00:05Titel
  • 01:18Cultivate L. pneumophila and Prepare and Quantify L. pneumophila Outer Membrane Vesicles (OMVs)
  • 03:42Pre-treat, Infect Macrophages, and Assess Bacterial Replication with a Colony-Forming Unit (CFU) Assay
  • 05:31Results: Pro-inflammatory Response of Macrophages from L. pneumophila Infection
  • 07:04Conclusion

Summary

Automatische Übersetzung

Her beskriver vi rensing av Legionella pneumophila (L. pneumophila) ytre membranblærer (OMVS) fra flytende kulturer. Disse rensede vesikler blir så brukt for behandling av makrofager for å analysere deres pro-inflammatorisk potensial.

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