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Schnelle Isolierung von BMPR-IB + Fettgewebe gewonnene Stromazellen für die Verwendung in einem Schädeldachdefektheilung Modell
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Rapid Isolation of BMPR-IB+ Adipose-Derived Stromal Cells for Use in a Calvarial Defect Healing Model
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Schnelle Isolierung von BMPR-IB + Fettgewebe gewonnene Stromazellen für die Verwendung in einem Schädeldachdefektheilung Modell

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09:07 min

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February 24, 2017

DOI:

10.3791/55120-v

DOI:
10.3791/55120-v

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09:07 min
February 24, 2017

•

1 Views
, , , , , , , , ,
1Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery,Stanford University School of Medicine

Summary

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Adipose-derived stromal cells may be useful for engineering new tissue from a patient's own cells. We present a protocol for the isolation of a subpopulation of human adipose-derived stromal cells (ASCs) with increased osteogenic potential, followed by application of the cells in an in vivo calvarial healing assay.

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Cite this Article

Marshall, C. D., Zielins, E. R., Brett, E. A., Blackshear, C. P., Hu, M. S., Leavitt, T., Barnes, L. A., Lorenz, H. P., Longaker, M. T., Wan, D. C. Rapid Isolation of BMPR-IB+ Adipose-Derived Stromal Cells for Use in a Calvarial Defect Healing Model. J. Vis. Exp. (120), e55120, doi:10.3791/55120 (2017).

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