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Virionlar antijenik Spectra ve ekstrasellüler veziküllerin Analiz Virometry Akış
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Kapitel
- 00:05Titel
- 01:09Magnetic Nanoparticle (MNP)-coupled Antibody Labeling and Particle of Interest Capture
- 02:33Magnetic Column MNP-captured Particle Unbound Antibody Separation and MNP-captured Particle Analysis
- 04:02Results: Representative Virion- and EV-capture Analyses
- 05:17Conclusion
Viruses or extracellular vesicles were immunocaptured with 15 nm magnetic nanoparticles coupled to antibodies recognizing surface antigens. The captured virions or vesicles were labeled with fluorescent antibodies against other surface antigens. The resultant complexes were separated in high magnetic field and analyzed with conventional flow cytometers triggered on fluorescence.
