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Entwicklungsbiologie

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诱导多能干细胞的快速神经元分化有关微电极阵列测量网络活动

JoVE Journal
Entwicklungsbiologie

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JoVE Journal Entwicklungsbiologie

Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays

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诱导多能干细胞的快速神经元分化有关微电极阵列测量网络活动

DOI:

10.3791/54900-v

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09:20 min

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January 08, 2017

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Monica Frega*^1,2, Sebastianus H. C. van Gestel*³, Katrin Linda³, Jori van der Raadt, Jason Keller², Jon-Ruben Van Rhijn², Dirk Schubert², Cornelis A. Albers^3,4, Nael Nadif Kasri^2,3

¹Department of Cognitive Neurosciences,Radboudumc, ²Donders Institute for Brain, Cognition and Behaviour,Radboud University, ³Department of Human Genetics,Radboudumc, ⁴Department of Molecular Developmental Biology,Radboud University

Kapitel

00:05Titel
00:41Plating hiPSCs
03:42Adding Rat Astrocytes
05:48Characterization of Neuronal Network Activity Using MEAs
06:31Results: Characterization of hiPSC-derived Neurons
08:21Conclusion

Summary

Automatische Übersetzung

我们修改和实施描述人类诱导多能干细胞（iPS细胞）的快速，可重复，高效分化成兴奋皮层神经元¹²先前公布的协议。具体地讲，我们的修改允许对神经元细胞密度和使用的控制上的微电极阵列以测量在网络级电生理特性。

Tags

Keywords: Neuronal Differentiation Induced Pluripotent Stem Cells Micro-electrode Arrays Neurological Disorders Neurobiology Rapid Differentiation DMEM/F12 CDS E8 Medium Doxycycline ROCK Inhibitor HiPSCs Cell Suspension Laminin MEAs Cell Plating

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