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Induzíveis linhas celulares estáveis marcado-LAP para investigar a função da proteína, Spatiotemporal Localização e redes de interação de proteínas

JoVE Journal
Biologie

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JoVE Journal Biologie

Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks

Induzíveis linhas celulares estáveis marcado-LAP para investigar a função da proteína, Spatiotemporal Localização e redes de interação de proteínas

9,598 Views

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11:04 min

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December 24, 2016

DOI:

10.3791/54870-v

DOI:

10.3791/54870-v

•

11:04 min

December 24, 2016

•

1 Views

Michelle Bradley, Ivan Ramirez, Keith Cheung, Ankur A. Gholkar, Jorge Z. Torres^2,3

¹Department of Chemistry and Biochemistry,University of California, Los Angeles, ²Molecular Biology Institute,University of California, Los Angeles, ³Jonsson Comprehensive Cancer Center,University of California, Los Angeles

Tags

Inducible LAP-tagged Stable Cell Lines Protein Function Spatiotemporal Localization Protein Interaction Networks HEK293 Cells Hygromycin Selection Tandem Affinity Purification (TAP)Tet/Dox Induction Anti-GFP Antibody Protein A Beads

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