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Индуцибельные LAP-меченый Стабильные клеточные линии для исследования белка функции, пространственно-временной локализации и белковое взаимодействие сетей
JoVE Journal
Biologie
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JoVE Journal Biologie
Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks
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Индуцибельные LAP-меченый Стабильные клеточные линии для исследования белка функции, пространственно-временной локализации и белковое взаимодействие сетей

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11:04 min

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December 24, 2016

DOI:

10.3791/54870-v

DOI:
10.3791/54870-v

•

11:04 min
December 24, 2016

•

2 Views
, , , ,
1Department of Chemistry and Biochemistry,University of California, Los Angeles, 2Molecular Biology Institute,University of California, Los Angeles, 3Jonsson Comprehensive Cancer Center,University of California, Los Angeles

Summary

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We describe a method for generating localization and affinity purification (LAP)-tagged inducible stable cell lines for investigating protein function, spatiotemporal subcellular localization and protein-protein interaction networks.

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Inducible LAP-taggedStable Cell LinesProtein FunctionSpatiotemporal LocalizationProtein Interaction NetworksHEK293 CellsHygromycin SelectionTandem Affinity Purification (TAP)Tet/Dox InductionAnti-GFP AntibodyProtein A Beads

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Bradley, M., Ramirez, I., Cheung, K., Gholkar, A. A., Torres, J. Z. Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks. J. Vis. Exp. (118), e54870, doi:10.3791/54870 (2016).

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