The social amoebae Dictyostelium discoideum has recently been established as a system to study protein misfolding and proteostasis. Here, we describe a new imaging-based methodology to study temperature-induced protein aggregation and the cellular stress response in D. discoideum.
Malinovska, L., Alberti, S. Studying the Protein Quality Control System of D. discoideum Using Temperature-controlled Live Cell Imaging. J. Vis. Exp. (118), e54730, doi:10.3791/54730 (2016).