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Utilizing the Ethylene-releasing Compound, 2-Chloroethylphosphonic Acid, as a Tool to Study Ethylene Response in Bacteria
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Biochemie
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JoVE Journal Biochemie
Utilizing the Ethylene-releasing Compound, 2-Chloroethylphosphonic Acid, as a Tool to Study Ethylene Response in Bacteria

Utilizing the Ethylene-releasing Compound, 2-Chloroethylphosphonic Acid, as a Tool to Study Ethylene Response in Bacteria

7,763 Views

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08:51 min

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November 10, 2016

DOI:

10.3791/54682-v

DOI:
10.3791/54682-v

•

08:51 min
November 10, 2016

•

1 Views
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1Molecular Microbial Biochemistry Laboratory, Faculty of Science (Applied Bioscience Program),University of Ontario Institute of Technology

Summary

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The protocols outlined herein facilitate the convenient investigation of bacterial ethylene responses by utilizing 2-chloroethylphosphonic acid (CEPA). Ethylene is produced in situ through the decomposition of CEPA in an aqueous bacterial growth medium, circumventing the requirement for pure ethylene gas.

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Cite this Article

Augimeri, R. V., Varley, A. J., Strap, J. L. Utilizing the Ethylene-releasing Compound, 2-Chloroethylphosphonic Acid, as a Tool to Study Ethylene Response in Bacteria. J. Vis. Exp. (117), e54682, doi:10.3791/54682 (2016).

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