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Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors

Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors

14,799 Views

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10:17 min

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October 09, 2016

DOI:

10.3791/54612-v

DOI:
10.3791/54612-v

•

10:17 min
October 09, 2016

•

17 Views
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1Department of Molecular Genetics,Weizmann Institute of Science, 2Electron Microscopy Unit,Weizmann Institute of Science

Summary

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This study presents the development of reproducible methodologies to study biofilm inhibitors and their effects on Bacillus subtilis multicellularity.

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B. Subtilis BiofilmsSmall Molecule Biofilm InhibitorsBiofilm FormationBacillus SubtilisPseudomonas AeruginosaXanthomonas CitriScanning Electron MicroscopyPellicle FormationBiofilm GrowthMSgg MediumStarter CultureOptical Density12-well Cell Culture PlateAgar Plate

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Bucher, T., Kartvelishvily, E., Kolodkin-Gal, I. Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors. J. Vis. Exp. (116), e54612, doi:10.3791/54612 (2016).

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