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Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors

Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors

DOI:
10.3791/54612-v

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10:17 min

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October 09, 2016

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, ,
1Department of Molecular Genetics,Weizmann Institute of Science, 2Electron Microscopy Unit,Weizmann Institute of Science

Kapitel

  • 00:05Titel
  • 01:15Preparation of Biofilm Pellicles and Colonies
  • 03:19Ethanol Resistance Assay
  • 05:17Biofilm Colony Preparation for Scanning Electron Microscopy (SEM)
  • 07:49Results: Bacillus subtilis Pellicle and Biofilm Formation Is Altered in the Presence of the Small Molecule Inhibitor D-leucine
  • 09:20Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This study presents the development of reproducible methodologies to study biofilm inhibitors and their effects on Bacillus subtilis multicellularity.

Tags

B. Subtilis BiofilmsSmall Molecule Biofilm InhibitorsBiofilm FormationBacillus SubtilisPseudomonas AeruginosaXanthomonas CitriScanning Electron MicroscopyPellicle FormationBiofilm GrowthMSgg MediumStarter CultureOptical Density12-well Cell Culture PlateAgar Plate

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