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मापने एंजाइम से जुड़ी Lectin परख द्वारा इन्फ्लुएंजा neuraminidase निषेध एंटीबॉडी titers
JoVE Journal
Immunologie und Infektion
This content is Free Access.
JoVE Journal Immunologie und Infektion
Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay
DOI:

08:11 min

September 06, 2016

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Kapitel

  • 00:05Titel
  • 00:39Determination of the Amount of Neuraminidase (NA) to Use in Enzyme-linked Lectin Assay (ELLA)
  • 04:10Enzyme-linked Lectin Assay (ELLA)
  • 06:15Results: Determination of the Neuraminidase Inhibition Titer
  • 07:40Conclusion

Summary

Automatische Übersetzung

We describe the enzyme-linked lectin assay (ELLA) for measuring influenza neuraminidase (NA)-inhibition antibody titers in sera. The assay uses peanut agglutinin to quantify galactose residues that become accessible when NA removes sialic acid from fetuin-coated, 96-well plates.

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