Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
L'utilisation de la Induced somatique analyse sectorielle (AISS) pour l'étude des gènes et de promoteurs impliqués dans la formation du bois et le développement de souches secondaire
8,664 Views
•
09:54 min
•
October 05, 2016
DOI:
1School of Ecosystem and Forest Sciences, Faculty of Science,The University of Melbourne, 2Victorian AgriBiosciences Centre,La Trobe University R&D Park, 3College of Biological Sciences, Department of Plant Biology,University of California, Davis, 4Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI),University of Pretoria
Summary
Automatically generated
Here we present a protocol that facilitates the medium to high throughput functional characterization of gene and promoter constructs in tree secondary stem tissue within comparatively short time frames. It is efficient, easy to use and widely applicable to a range of tree species.
Read Article
Cite this Article
Spokevicius, A., Taylor, L., Melder, E., Van Beveren, K., Tibbits, J., Creux, N., Bossinger, G. The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development. J. Vis. Exp. (116), e54553, doi:10.3791/54553 (2016).
Copy