Journal
/
Chemie
/
Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
JoVE Journal
Chemie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Chemie
Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time

DOI:
10.3791/54549-v

•

11:05 min

•

October 26, 2016

•

, , ,
1Molecular Medicine Research Group,Western Sydney University, 2Australian Centre for Research on Separation Science,Western Sydney University, 3School of Science and Health,Western Sydney University, 4School of Medicine,Western Sydney University

Kapitel

  • 00:05Titel
  • 00:50Preparation of Chitosan Films
  • 02:23Preparation and Validation of the Capillary Electrophoresis Instrument
  • 06:25Grafting of RGDS onto Chitosan Film
  • 08:52Results: Reduction of RGDS during Grafting Reaction onto Chitosan Film
  • 10:15Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Free solution capillary electrophoresis is a fast, cheap and robust analytical method that enables the quantitative monitoring of chemical reactions in real time. Its utility for rapid, convenient and precise analysis is demonstrated here through analysis of covalent peptide grafting onto chitosan films for improved cell adhesion.

Tags

Capillary ElectrophoresisPeptide GraftingChitosan FilmsReal-time MonitoringAnalytical ChemistryPolymer ChemistryDrug BindingDigestionAcetic AcidCentrifugationFilm PreparationCapillary PreparationFused Silica Capillary

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates

Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles

Cooling Rate Dependent Ellipsometry Measurements to Determine the Dynamics of Thin Glassy Films

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry

Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds

Grafting Multiwalled Carbon Nanotubes with Polystyrene to Enable Self-Assembly and Anisotropic Patchiness

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog (Xenopus laevis) Embryos

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Read Article