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Artsbestemmelse og kvantificering i blandinger Brug MRM massespektrometri af peptider Anvendt til Kød Authentication
JoVE Journal
Biochemie
This content is Free Access.
JoVE Journal Biochemie
Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
DOI:

09:26 min

September 20, 2016

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Kapitel

  • 00:05Titel
  • 00:54Proteolysis and Analysis of Reference Myoglobins
  • 05:14Preparation and Analysis of Calibration Samples
  • 07:57Results: Example Outcomes for a Mixture of Horse in Beef
  • 08:33Conclusion

Summary

Automatische Übersetzung

We present a protocol for identifying and quantifying the components in mixtures of species possessing similar proteins. Mass spectrometry detects peptides for identification, and gives relative quantitation by ratios of peak areas. As a tool food for fraud detection, the method can detect 1% horse in beef.

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