Journal
/
Neurowissenschaften
/
Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy
JoVE Journal
Neurowissenschaften
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Neurowissenschaften
Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy

Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy

DOI:
10.3791/54214-v

•

07:47 min

•

July 09, 2016

•

, , , , ,
1Virginia Tech Carilion Research Institute, 2Renovo Neural Incorporated

Kapitel

  • 00:05Titel
  • 00:54Brain Tissue Preparation
  • 03:32SBFSEM Imaging
  • 04:15Analyzing the Imaging Dataset
  • 05:43Results: Analysis of Brain Mitochondrial Morphology, Distribution, Abundance and Ultrastructure Using Serial Block-Face Scanning Electron Microscopy
  • 07:04Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Mitochondrial visualization and analysis from mammalian brain tissue is a challenging task. Here, we describe how three dimensional (3D) reconstruction analysis from the serial block-face scanning electron microscopy (SBFSEM) can be used to gain insights on the morphological and volumetric analysis of this critical energy generating organelle.

Tags

Keywords: Brain MitochondriaSerial Block-face Scanning Electron MicroscopyMitochondrial MorphologyNeurodegenerative DisordersNeurodevelopmental DisordersSample PreparationStainingDehydrationEmbedding

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

check_url/de/54214?article_type=v

Studying Synaptic Vesicle Pools using Photoconversion of Styryl Dyes
check_url/de/54214?article_type=v

Intravital Microscopy of the Mouse Brain Microcirculation using a Closed Cranial Window
check_url/de/54214?article_type=v

Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue
check_url/de/54214?article_type=v

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI
check_url/de/54214?article_type=v

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration
check_url/de/54214?article_type=v

The Olfactory System as a Model to Study Axonal Growth Patterns and Morphology In Vivo
check_url/de/54214?article_type=v

Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers
check_url/de/54214?article_type=v

Peering at Brain Polysomes with Atomic Force Microscopy
check_url/de/54214?article_type=v

Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy
check_url/de/54214?article_type=v

Chronic Implantation of Whole-cortical Electrocorticographic Array in the Common Marmoset

Read Article