/
/
Çizgili Deneyi Disosiye Hipokampal Nöronlar kullanarak Protein Substrat çekici veya itici Aktivite Eğitim için
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Çizgili Deneyi Disosiye Hipokampal Nöronlar kullanarak Protein Substrat çekici veya itici Aktivite Eğitim için
10,391 Views
•
08:11 min
•
June 19, 2016
DOI:
Summary
Automatically generated
Axon guidance molecules regulate neuronal migration and targeted growth-cone navigation. We present a powerful method, the stripe assay, to assess the ability of guidance molecules to attract or repulse neurons. In this protocol, we demonstrate the stripe assay by showing FLRT2's ability to repel cultured hippocampal neurons.
Read Article
Cite this Article
Yamagishi, S., Kesavamoorthy, G., Bastmeyer, M., Sato, K. Stripe Assay to Study the Attractive or Repulsive Activity of a Protein Substrate Using Dissociated Hippocampal Neurons. J. Vis. Exp. (112), e54096, doi:10.3791/54096 (2016).
Copy