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Murine Hind Limb Long Bone Dissection og benmarg Isolation
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation
DOI:

07:17 min

April 14, 2016

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Kapitel

  • 00:05Titel
  • 00:41Hind Limb Long Bone Dissection
  • 03:01Long Bone Preparation For Bone Marrow Isolation
  • 04:25Bone Marrow Isolation
  • 05:29Results: Representative Downstream Analyses and Applications of Rapid DIssected Mouse Long Bones and Bone Marrow
  • 06:28Conclusion

Summary

Automatische Übersetzung

Here we present a protocol for the dissection of hind limb long bones (femurs and tibiae) from the laboratory mouse. We further describe a rapid technique for bone marrow isolation from these bones that utilizes centrifugation for removal of bone marrow from the bone marrow space.

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