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Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli
JoVE Journal
Biologie
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JoVE Journal Biologie
Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli

DOI:
10.3791/53618-v

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08:25 min

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March 20, 2016

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, , ,
1U1001,Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris Descartes

Kapitel

  • 00:05Titel
  • 00:40Site Directed Mutagenesis on sRNA Expression Cassette
  • 03:40Production and Harvest of M13-packaged Phagemid Stocks
  • 05:01Silencing by Infection with Packaged Phagemids
  • 06:15Results: Gene Silencing in E. coli Using sRNA Phagemids
  • 07:36Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

We describe a method to knock down gene expression in a growing population of E. coli cells using sequence-targeted sRNA expression cassettes delivered by an M13 phagemid vector.

Tags

Phage-mediated DeliveryTargeted SRNA ConstructsGene KnockdownE. ColiSite-directed MutagenesisSRNA Expression CassetteGuide SequencePCRDpnI DigestionChemical TransformationColony PCRGene SilencingSynthetic BiologyVirulence Factors

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