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Kapitel
- 00:05Titel
- 00:27Fmoc-protected Amino Acid Coupling
- 02:03Anhydride/Acid Coupling
- 03:01N-methyltrityl (Mtt) Protecting Group Deprotection
- 04:18Cyclization of the Linear Peptide
- 04:57Cleavage and Deprotection of Side-chain Groups
- 06:04Kaiser (Ninhydrin) Test
- 07:01Results: Synthesis of Cyclic Peptides Using Microwave Irradiation
- 07:45Conclusion
A simple and general method for the synthesis of cyclic peptides using microwave irradiation is outlined. This procedure enables the synthesis of backbone cyclic peptides with a collection of different conformations while retaining the side chains and the pharmacophoric moieties., and therefore, allows to screen for the bioactive conformation.
Tags
Backbone Cyclic Peptide LibraryAntiparasitic TherapeuticsMicrowave IrradiationProtein-protein InteractionsAmino AcidActivatorActivator BasePolypropylene CartridgeCoupling ReactionNN-dimethylformamidePiperidine1-hydroxybenzotriazoleDichloromethane1-methyl-2-pyrrolidinoneAnhydride4-DimethylaminopyridineNN-diisopropylethylamineTrifluoroacetic AcidTriisopropylsilane
