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Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells

Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells

DOI:
10.3791/53408-v

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12:16 min

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December 17, 2015

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,
1Philips Institute for Oral Health Research,Virginia Commonwealth University, 2Department of Microbiology and Immunology,Virginia Commonwealth University, 3Department of Biochemistry,Virginia Commonwealth University

Kapitel

  • 00:05Titel
  • 01:15Anaerobic Bacteria Infection Assay
  • 05:07Survival of Internalized Bacteria
  • 06:15Fluorescence Microscopy of Internalized Bacteria
  • 09:15Results: Infection of HUVECs with Porphyromonas Gingivalis
  • 10:46Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This article presents two protocols: one to measure anaerobic bacteria that can successfully invade and survive within the host, and the other to visualize anaerobic bacteria interacting with host cells. This study can be applied to any cultivable anaerobe and any eukaryotic cell type.

Tags

Porphyromonas GingivalisAnaerobic BacteriaHost Cell InvasionAntibiotic Protection AssayFluorescent MicroscopyBCECF-AMTRITC PhalloidinDAPIZ-stack Imaging

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