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Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer
JoVE Journal
Biotechnik
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JoVE Journal Biotechnik
Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer

Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer

7,867 Views

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07:54 min

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October 15, 2015

DOI:

10.3791/53376-v

DOI:
10.3791/53376-v

•

07:54 min
October 15, 2015

•

6 Views
, , ,
1Department of Bio and Brain Engineering,KAIST, 2Information and Electrical Research Institute,KAIST

Summary

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We present a new technique to measure the lateral diffusion of a surface active species at the fluid-fluid interface by merging a droplet monolayer onto a flat monolayer.

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Fluorescence RecoveryMerging DropletTwo-dimensional DiffusionPhospholipid MonolayerFRAMFRAPSurfactant MonolayerFluid-fluid InterfaceLateral DiffusivityCoalescenceFluorescence MicroscopeDye MoleculesDiffusion Area

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Cite this Article

Jeong, D., Kim, K., Choi, M. C., Choi, S. Q. Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer. J. Vis. Exp. (104), e53376, doi:10.3791/53376 (2015).

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