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Métodos de Transferência Precisamente localizada de células ou DNA em adiantado postimplantation embriões de camundongos
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Métodos de Transferência Precisamente localizada de células ou DNA em adiantado postimplantation embriões de camundongos
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December 25, 2015
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We demonstrate a method for grafting cultured cells into defined sites of early mouse embryos to determine their in vivo potential. We also introduce an optimized electroporation method that uses glass capillaries of known diameter, allowing the precise delivery of exogenous DNA into a few cells in the embryos.
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Huang, Y., Wilkie, R., Wilson, V. Methods for Precisely Localized Transfer of Cells or DNA into Early Postimplantation Mouse Embryos. J. Vis. Exp. (106), e53295, doi:10.3791/53295 (2015).
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