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DNA, Oksidasyon Biomarker HPLC ölçümü, 8-okso-7,8-dihidro-2'-deoksiganozin, Kültürlenmiş Hücrelerde ve hayvansal dokular
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HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues
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DNA, Oksidasyon Biomarker HPLC ölçümü, 8-okso-7,8-dihidro-2'-deoksiganozin, Kültürlenmiş Hücrelerde ve hayvansal dokular

25,985 Views

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14:12 min

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August 01, 2015

DOI:

10.3791/52697-v

DOI:
10.3791/52697-v

•

14:12 min
August 01, 2015

•

7 Views
, , , , , ,
1Environmental Health Science and Research Bureau,Health Canada

Summary

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Bu protokolün amacı bir DNA oksidasyonu marker algılama, 8-okso-7,8-dihidro-2'-deoksiganozin kültürlenmiş hücreler ya da hayvan dokularından DNA (8-okso-dGuo) olarak hazırlanmıştır HPLC-ED ile.

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Cite this Article

Chepelev, N. L., Kennedy, D. A., Gagné, R., White, T., Long, A. S., Yauk, C. L., White, P. A. HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues. J. Vis. Exp. (102), e52697, doi:10.3791/52697 (2015).

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