Journal
/
Immunologie und Infektion
/
عزل الخلايا اللمفاوية من الجلد البشري لتحليل المظهري و<em> فيفو السابقين</em> زراعة الخلايا
JoVE Journal
Immunologie und Infektion
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Immunologie und Infektion
Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
Please note that all translations are automatically generated. Click here for the English version.

عزل الخلايا اللمفاوية من الجلد البشري لتحليل المظهري و<em> فيفو السابقين</em> زراعة الخلايا

14,268 Views

•

10:31 min

•

April 08, 2016

DOI:

10.3791/52564-v

DOI:
10.3791/52564-v

•

10:31 min
April 08, 2016

•

9 Views
, , , ,
1Laboratory of Medical Immunology, Department of Laboratory Medicine,Radboud University Medical Centre, 2Department of Dermatology,Radboud University Medical Centre

Summary

Automatically generated

We describe a protocol to efficiently isolate skin resident T cells from human skin biopsies. This protocol yields sufficient numbers of viable human skin resident lymphocytes for flow cytometric analysis and ex vivo culture.

Explore More Videos

Lymphocyte IsolationSkin BiopsyT CellsFlow CytometryEx Vivo Cell CultureSingle Cell Suspension

Read Article

Cite this Article

He, X., de Oliveira, V. L., Keijsers, R., Joosten, I., Koenen, H. J. Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture. J. Vis. Exp. (110), e52564, doi:10.3791/52564 (2016).

Download .ris file

Copy

Share Video

.

Copy