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El bioconjugación y Radiosíntesis de<sup> 89</sup> Los anticuerpos marcados con Zr-DFO
JoVE Journal
Chemie
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JoVE Journal Chemie
The Bioconjugation and Radiosynthesis of 89Zr-DFO-labeled Antibodies
DOI:

09:29 min

February 12, 2015

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Kapitel

  • 00:05Titel
  • 01:15Conjugation of DFO-NCS to J591
  • 02:56Radiolabeling J591-DFO with 89Zr
  • 06:16In Vivo PET Imaging with 89Zr-DFO-J591
  • 07:52Results: PET Imaging with 89Zr-DFO-J591
  • 09:03Conclusion

Summary

Automatische Übersetzung

Due to its multi-day radioactive half-life and favorable decay properties, the positron-emitting radiometal 89Zr is extremely well-suited for use in antibody-based radiopharmaceuticals for PET imaging. In this protocol, the bioconjugation, radiosynthesis, and preclinical application of 89Zr-labeled antibodies will be described.

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