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Developmental Biology

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JoVE Journal Developmental Biology
In Utero Intra-cardiac Tomato-lectin Injections on Mouse Embryos to Gauge Renal Blood Flow
 

In Utero Intra-cardiac Tomato-lectin Injections on Mouse Embryos to Gauge Renal Blood Flow

Article DOI: 10.3791/52398-v • 10:25 min • February 4th, 2015
February 4th, 2015 •
1,2, 1,2
1Rangos Research Center, Children's Hospital of Pittsburgh of UPMC, 2Division of Nephrology, Department of Pediatrics, University of Pittsburgh School of Medicine

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Summary
Automatic Translation

This manuscript describes a technique for visualization of the developing vasculature. Here we utilized in utero intra-cardiac FITC-labeled tomato lectin microinjections on mouse embryos. Using this technique, we delineate the perfused and unperfused vessels throughout the embryonic kidney.

Tags

In Utero Intra-cardiac Tomato-lectin Injections Mouse Embryos Renal Blood Flow Formation Perfusion Developing Renal Blood Vessels Glomeruli Vasculature Angiogenesis Vasculogenesis Perfusion Mapping Techniques Resin Casts In Vivo Ultrasound Imaging Micro-dissection Intimate Relationships Renal Structures In Utero Intra-cardiac Ultrasound-guided FITC-labeled Tomato Lectin Microinjections Ontogeny Of Renal Perfusion Tissues Co-stained Nephron Progenitors Nephron Structures Ureteric Epithelium Large Caliber Vessels Peripheral Vessels Glomeruli Experimental Technique
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