/
/
In Utero Intra-cardiac Tomato-lectin Injections on Mouse Embryos to Gauge Renal Blood Flow
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Kapitel
- 00:05Titel
- 01:16Preparations
- 03:22Extracting the Embryos
- 05:49Injecting the Embryos
- 08:29Results: Vascular Formation Precedes Flow in the Developing Kidney
- 10:01Conclusion
This manuscript describes a technique for visualization of the developing vasculature. Here we utilized in utero intra-cardiac FITC-labeled tomato lectin microinjections on mouse embryos. Using this technique, we delineate the perfused and unperfused vessels throughout the embryonic kidney.
